H-MAPD: Human MLPA Probe Design

Sequence (FASTA format)
Hybridization temperature°C
[Na+]M
[Mg2+]M
GC% range for hybridizing sequences
Min. free energy change (ΔG) allowed kcal/mol
Max. perfect matches allowed in genome
Max. repeat sequence match allowed nt
Protocol:  Electrophoresis based
FlexMAP bead coupled
Use Stuffer Sequence  No Stuffer Sequence
Bead tag in Left Probe  Bead tag in Right Probe
Left primer sequence (5' -> 3')
Right primer sequence (5' -> 3')
(Min.) Length of ligation product nt
Email address to receive result
 
    
Conditions of Use
•The H-MAPD software is free for non-commercial use. Commercial users should contact:
      Jizu Zhi (jzhi@ic.sunysb.edu) or Eli Hatchwell (eli.hatchwell@stonybrook.edu)
      Genomics Core Facility, Stony Brook University
•Since the software utilizes the UCSC genome browser and UNAFold, commercial users also need to obtain a licence for those programs.
•The sequence input takes maximum 100,000 bases.
Citation
Zhi J, Hatchwell E (2008) Human MLPA Probe Design (H-MAPD): a probe design tool for both electrophoresis-based and bead-coupled human multiplex ligation-dependent probe amplification assays. BMC Genomics 9: 407. [Link]